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metabolomics services DATA SCIENCE
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Our services encompass a wide range of analytical platforms and specialized sample preparation methods. Using UPLC-MS technology, we offer selective and sensitive metabolomics analyses, providing comprehensive coverage of the metabolome. Partner with us to gain valuable insights into the molecular landscape that drives health and disease. Our advanced analytical platforms utilize liquid chromatography interfaced mass spectrometers, perfectly suited for metabolomics applications. We analyze samples in positive and/or negative electrospray ionization (ESI) modes, scanning from m/z 50-1200.
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All Quantitative platforms Semiquantitative platforms

Lipidomics-Platform 1

Lipids play essential roles in biological systems, such as forming cell membranes, providing a hydrophobic medium for membrane proteins, and participating in signal transduction pathways. This platform utilizes methanol extraction and the analysis is performed by UHPLC coupled to a Time-of-Flight (ToF) mass spectrometer, allowing the analysis of the following lipid species:

⦁ Fatty acyls: non esterified fatty acids, oxidised fatty acids, N-acyl ethanolamines and acylcarnitines.
⦁ Sterol lipids: bile acids and steroids.
⦁ Glycerophospholipids (lyso-forms): lysophosphatidylethanolamines, lysophosphatidylinositols, lysophosphatidylglycerols and lysophosphatidylcholines, including ether-linked species.
⦁ Free sphingoid bases.

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Lipidomics-Platform 1

Fatty acyls: non esterified fatty acids, oxidised fatty acids, N-acyl ethanolamines and acylcarnitines.

Sterol lipids: bile acids and steroids.

Glycerophospholipids (lyso-forms): lysophosphatidylethanolamines, lysophosphatidylinositols, lysophosphatidylglycerols and lysophosphatidylcholines, including ether-linked species.

Free sphingoid bases.



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Lipidomics – Platform 2

Like Platform 1, this platform focuses on lipid analysis, employing chloroform/methanol extraction and the analysis is performed by UHPLC coupled to a Time-of-Flight (ToF) mass spectometer. This platform covers:

⦁ Fatty acyls: primary fatty amides.
⦁ Sterol lipids: Cholesterol esters, cholesterol and cholesteryl sulfate.
⦁ Sphingolipids: ceramides, monohexosylceramides and sphingomyelins.
⦁ Glycerolipids: monoacylglycerols, diacylglycerols and triacylglycerols.
⦁ Glycerophospholipids: phosphatidylethanolamines, phosphatidylinositols, and phosphatidylcholines, including ether-linked species.

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Lipidomics – Platform 2

Fatty acyls: primary fatty amides.

Sterol lipids: Cholesterol esters, cholesterol and cholesteryl sulfate.

Sphingolipids: ceramides, monohexosylceramides and sphingomyelins.

Glycerolipids: monoacylglycerols, diacylglycerols and triacylglycerols.

Glycerophospholipids: phosphatidylethanolamines, phosphatidylinositols, and phosphatidylcholines, including ether-linked species.



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Amino acids – Platform 3

This platform analyses amino acids, the building blocks of proteins and metabolic intermediates. Using a derivatization method on methanol extracts it detects:

⦁ Amino acids.
⦁ Di- and tripeptides.
⦁ Amino acids derivatives.

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Amino acids – Platform 3

Amino acids.

Di- and tripeptides.

Amino acids derivatives.



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Polar metabolites – Platform 4

Focused on polar metabolites, this platform identifies metabolites from central carbon metabolic pathways

⦁ Pentose Phosphate Pathway, TCA cycle and glycolysis.
⦁ Carboxylic acids
⦁ Nucleosides and nucleotides
⦁ Vitamins

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Polar metabolites – Platform 4

Pentose Phosphate Pathway, TCA cycle and glycolysis.

Carboxylic acids

Nucleosides and nucleotides

Vitamins



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Bile acids – Platform 5

Bile acids, amphiphilic molecules derived from cholesterol, aid in the absorption and transport of lipids, nutrients, and vitamins, and serve as signaling molecules. This platform covers:

⦁ Free bile acids
⦁ Tauroconjugated bile acids
⦁ Glycoconjugated bile acids

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Bile acids – Platform 5

Free bile acids

Tauroconjugated bile acids

Glycoconjugated bile acids



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Oxidized fatty acids – Platform 6

Focusing on lipophilic signaling molecules derived from the oxidation of polyunsaturated fatty acids, this platform analyzes

⦁ Oxidized fatty acids derived from omega-6 fatty acids (arachidonic acid, linoleic acid, dihomo-γ-linoleic acid)
⦁ Oxidized fatty acids derived from omega-3 fatty acids (α-linoleic acid, docosahexaenoic acid, eicosapentaenoic acid)

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Oxidized fatty acids – Platform 6

Oxidized fatty acids derived from omega-6 fatty acids (arachidonic acid, linoleic acid, dihomo-γ-linoleic acid)

Oxidized fatty acids derived from omega-3 fatty acids (α-linoleic acid, docosahexaenoic acid, eicosapentaenoic acid)



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Additional tailor-made analyses

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Additional tailor-made analyses

We provide customized analysis proposals for other metabolites or quantifications on a case-by-case basis. Extensive data analysis, including correlation studies and predictive algorithms, can also be performed upon request.

Each platform also includes unidentified analytes that contribute significantly to metabolic phenotypes. Our continual review and utilization of new MS/MS and complementary analytical data ensure up-to-date information on potential metabolite identifications.



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Advanced Analytics

DATA SCIENCE

OWL Metabolomics stands at the forefront of comprehensive data-driven insights and rigorous analysis in metabolic profiles. As we know that data is your greatest asset, we bring together a team of skilled data scientists who collaborate closely with researchers. Our work pursues empowering clients in harnessing the full potential of their data and gaining profound insights into metabolic profiles.

We leverage innovative data science techniques to identify significant biomarkers, perform comprehensive multivariate analyses, model intricate metabolic pathways, and conduct robust data integration. By tailoring our advanced and rigorous data analysis methods to our clients specific metabolomic needs, we empower them to drive research and enhance personalized medicine through impactful data-driven discoveries.

Our Key Data Science Services in Metabolomics

We offer a complete range of metabolomic and data science services to satisfy your investigation and analysis needs. Using our expertise we to transform data without processing into available scientific knowledge, through the following services:

Exploratory Data Analysis

With meticulous planning and design of experiments, we aim to analyze the data obtained to test your hypothesis with appropriate levels of statistical power and sensitivity.

Data Preparation

We understand that tidy data is the key for high-quality analysis. Our services include data cleaning and homogenizing of data sources, which are necessary tasks for producing reproducible research and comparable experiments.

Statistical Design

We are committed to extracting the maximum value from your data through exploratory and statistical analysis, aiding in the discovery of patterns that could further support your hypothesis.

Predictive Modelling

We transform it into high-performance predictive models that are reliable for creating new data, helping in the development of new products and services.

Communication

We support your communication for reports and publications, by providing formal explanations, high-quality graphics, data applications, and interactive documents.

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By Rubió
Carrer Indústria 29, Polígon Industrial Comte de Sert 08755
Castellbisbal, Barcelona (España), +34 937 722 509labrubio@labrubio.com
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